The <a href="https://www.medchemexpress.com/Abrocitinib.html">PF-04965842
JAK</a> publication fund of the University of Gottingen and the Deutsche Forschungsgemeinschaft supported open access publication of this article. The funders had no role in study <a href="https://www.medchemexpress.com/Abrocitinib.html">Abrocitinib
Cancer</a> design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: The authors have declared that no competing interests exist.(< 10 M; ) because it is bound to soil particles, to organic compounds, and forms precipitates with other soil elements like iron (Fe), aluminum (Al) and calcium (Ca) . To acquire and translocate phosphate, plants possess a number of different phosphate transporters (PHTs). In Arabidopsis thaliana, plasma membrane located PHTs of family 1 are responsible for P uptake into the root and show strong up-regulation under P deprivation [4,5]. In poplar, the expression of orthologs of the AtPHT1 genes is also strongly increased upon P starvation . Furthermore, the expression of distinct purple acid phosphatases is enhanced under P deficiency . Phosphatases and organic acids, which are secreted by the roots, increase phosphate availability in soil and together with enhanced P uptake capacities, P acquisition is increased under P starvation [7,10]. Altogether adaptation to limiting P supply results in reduced growth, but higher P use efficiency [6,7]. P deficiency affects not only P uptake and utilization, but also other plant nutrients. For example, in Arabidopsis P starvation caused decreased potassium (K) and enhanced iron (Fe) concentration in the leaves . Furthermore, reductions in calcium (Ca), magnesium (Mg) and manganese (Mn) were detected; it was speculated that these changes may indicate an adjustment of the ionic charge [11,13] or could be the result of reduced P availability in common P requiring steps of metabolism and transport . There are obviously interrelations among processes that regulate plant nutrient balance because deprivation of tomato in either P or K or Fe resulted in rapid and partly overlapping transcriptional responses . In Arabidopsis, a number of genes, which are highly regulated in response to P starvation [12,157], are also required for the utilization of other elements, e.g., SULTR1;3 for sulfate transport and NAS1 for iron chelation by nicotianamine [18,19]. In poplar, reduced P availability leads to higher nitrogen (N) concentrations in roots and lower N concentrations in leaves with higher free amino acid concentrations and reduced activities of N-related enzymes (nitrate reductase (NR), glutamine oxoglutarate aminotransferase (GOGAT), glutamine dehydrogenase (GDH); [6,7]). However, comprehensive analyses how P deficiencies affect the tissue concentrations of essential nutrient elements in poplar and whether these changes are related to alterations in the transcriptome are lacking. The main goal of this study was to characterize changes in nutrient element concentrations (P, S, C, N, K, Mg, Ca, Fe, Zn, Mn, and the non-essential element Al) in response to P starvation in poplar (Populus canescens) and to differentiate between transcriptomic changes directly correlated with the plant P concentrations and transcriptomic changes linked to down-stream changes of other nutrient concentrations. For this purpose, we studied poplar transcriptomes under high, intermediate and low P availabilities in leaves and roots, determined weighted gene co-expression networks.Ion" provided financial support for this research Project under grant number Po362/22-2. The publication fund of the University of Gottingen and the Deutsche Forschungsgemeinschaft supported open access publication of this article.