Lises with active JNK on the cytoskeleton (Fig 4C). In addition

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B) Quantification shows that Anisomicin treatment and JNK expression <a href="http://www.chemscene.com/189453-10-9.html">MedChemExpress CS-0655</a> increase the level of MT stability in neurons C) Dvl-expressing neurons treated with the JNK <a href="http://www.chemscene.com/775304-57-9.html">775304-57-9 site</a> inhibitor SP600125 or expressing the dominant negative JNK (JBD) show decreased MT stability when compared to control Dvl-expressing neurons. Three asterisks, P < 0.001; For asterisks, P < 0.0001.Page 6 of(page number not for citation purposes)BMC Cell Biology 2007, 8:http://www.biomedcentral.com/1471-2121/8/Figure 4 Dvl expression <a href="http://www.chemscene.com/122111-03-9.html">purchase 122111-03-9</a> activates JNK <a href="https://www.ncbi.nlm.nih.gov/pubmed/28549178" title=View Abstract(s)">PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28549178</a> Dvl expression activates JNK. Two asterisks, P < 0.01.Page 7 of(page number not for citation purposes)BMC Cell Biology 2007, 8:http://www.biomedcentral.com/1471-2121/8/The DEP domain of Dishevelled is dispensable for signalling to JNK The Dvl protein has three main conserved domains that are required for signalling through different branches of the Wnt pathway [37,38]. Many studies have shown that DIX and PDZ domains of Dvl are required for the canonical Wnt pathway whereas the DEP domain is required for the PCP pathway [31,39]. However, the correlation between domains and the activation of specific Wnt pathways has proved to be more complex. For example, in some systems the DEP domain is required for the canonical pathway [37,39]. Similarly, the PDZ domain can activate the non-canonical pathway [26,31,38]. In the divergent canonical pathway, the PDZ domain is required for Dvl-Gsk3 mediated MT stability [4]. To begin to understand how Dvl signals through JNK, we have examined the contribution of the different domains of Dvl on JNK-mediated MT stability.Lises with active JNK on the cytoskeleton (Fig 4C). In addition, Dvl expression increases the level of p-JNK bound to the cytoskeleton when compared to EGFP transfected control cells (Fig 4C). These results strongly suggest that Dvl increases the level of active JNK associated with MTs.Page 5 of(page number not for citation purposes)BMC Cell Biology 2007, 8:http://www.biomedcentral.com/1471-2121/8/Figure 3 Activation of JNK induces MT stability while inhibition of JNK blocks MT stability by Dvl Activation of JNK induces MT stability while inhibition of JNK blocks MT stability by Dvl. A) EGFP-expressing control neurons treated with low concentration of Anisomicin show higher level of microtubule stability when compared to EGFPexpressing neurons. Neurons expressing JNK alone reveals a level of stability comparable to that one obtained after exposure of neurons to Anisomicin. B) Quantification shows that Anisomicin treatment and JNK expression increase the level of MT stability in neurons C) Dvl-expressing neurons treated with the JNK inhibitor SP600125 or expressing the dominant negative JNK (JBD) show decreased MT stability when compared to control Dvl-expressing neurons. D) Quantification shows that treatment of Dvl expressing neurons with JNK inhibitor SP 600125 or expression of JBD reduces the level of MT stability. Scale bar 15 m. Three asterisks, P < 0.001; For asterisks, P < 0.0001.Page 6 of(page number not for citation purposes)BMC Cell Biology 2007, 8:http://www.biomedcentral.com/1471-2121/8/Figure 4 Dvl expression activates JNK <a href="https://www.ncbi.nlm.nih.gov/pubmed/28549178" title=View Abstract(s)">PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28549178</a> Dvl expression activates JNK. A) Induction of Dvl-ER expression for three hours with -estradiol increases the level of pc-Jun, a read-out of endogenous JNK activity, when compared to controls. Low levels of <a href="https://www.ncbi.nlm.nih.gov/pubmed/28536850" title=View Abstract(s)">PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28536850</a> Anisomicin also increase the level of p-c-Jun when compared with control untreated EGFP-expressing neurons or neurons exposed to -estradiol for three hrs. However, the levels of total c-Jun remain unchanged. B) Quantification of three independent experiments shows that induction of Dvl-ER expression induces a 40  increase in p-c-Jun levels compare to control. C) Expression of Dvl increases the level of p-JNK decorating the MT network along neurites when compared to EGFP-expressing control neurons.

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