Arison and Student t test for between-group comparison. All data were

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The morphometric analysis revealed no statistical difference (p > 0.05) in the number of Leydig cells expressed during the two distinct <a href="http://www.chemscene.com/2101955-00-2.html">2101955-00-2 site</a> phases of <a href="http://www.chemscene.com/1936529-65-5.html">CS-6458 web</a> reproductive cycle.In the testis, seminiferous tubules showed active spermatogenesis only in the reproductive ducks when Sertoli cells were more visible. The immunohistochemical tests detected D-Asp and NOS positive materials <a href="http://www.chemscene.com/139504-50-0.html">purchase Mertansine</a> mostly in Leydig cells. These cells were usually well distinguishable because of their larger volume and roundish shape. In the intertubular spaces, they were isolated or grouped in clusters with a variable number of components. The morphometric analysis revealed no statistical difference (p > 0.05) in the number of Leydig cells expressed during the two distinct phases of reproductive cycle.In the testis, seminiferous tubules showed active spermatogenesis only in the reproductive ducks when Sertoli cells were more visible. The immunohistochemical tests detected D-Asp and NOS positive materials mostly in Leydig cells. A <a href="https://www.ncbi.nlm.nih.gov/pubmed/28478730" title=View Abstract(s)">PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28478730</a> feeble positive reaction was also noticed in Sertoli cells, in peritubular cells surrounding the seminiferous tubule, in spermatogonia and in spermatids. No differences in their staining pattern were observed during either period (description not given). Differences in the number of Leydig cells involved and in their staining intensity to D-Asp and NOS immunoreactive methods were observed in animals deriving from both phases of reproductive cycle. Indeed, whereas NOS staining intensity <a href="https://www.ncbi.nlm.nih.gov/pubmed/25155999" title=View Abstract(s)">PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25155999</a> was mainly greater during NRP, D-Asp immunoreactivity was greater during RP. Figure 1 reports D-Asp immunoreactive distribution in testes of ducks belonging to the RP (Figure. 1A and 1B) and to the NRP (Figure. 1C). D-Asp immunoreactivity was primarily found in Leydig cells (Figure. 1A and 1B). The positive Leydig cells were more numerous and mainly grouped in clusters during the RP (Figure. 1A). Figure 1BPage 4 of(page number not for citation purposes)Reproductive Biology and Endocrinology 2008, 6:http://www.rbej.com/content/6/1// /,PPXQRSRVLWLYH /H\GLJ FHOOV PP'Figure 1 reproductive period (RP) (A and B) and the non reproductive period (NRP) (C) testes of the duck A. platyrhynchos during the Representative immunohistochemical distribution and localization of D-Asp in the Representative immunohistochemical distribution and localization of D-Asp in the testes of the duck A. platyrhynchos during the reproductive period (RP) (A and B) and the non reproductive period (NRP) (C). D-Asp immunopositivity was mainly localized in Leydig cells of the RP (A and B) and completely absent in the NRP (C). Histograms represent quantification of immunopositive Leydig cells/mm2 (D). **, p < 0.01 RP versus NRP. L, lumen. Scale bars: a, 50 m; b, 20 m; c, 100 m.shows a section at higher magnification of immunopositive Leydig cell types. During the NRP (Figure. 1C), reactive Leydig cells were present only in few of the examined sections and, when present, they appeared isolated. The morphometric analysis (Figure. 1D) revealed the quantification of immunopositive Leydig cells/mm2 during both periods: immunopositivity was higher during the RP than during the NRP (p < 0.01). NOS immunoreactive distribution in the testes of ducks belonging to the RP (Figure. 2A) and to the NRP (Figure. 2B and 2C) is shown in Figure.

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